Researchers at the Brown Medical School have figured out the fate of healthy protein when it comes in contact with the infectious prion form in yeast: the protein converts to the prion form, rendering it infectious.

Prions are infectious proteins that cause fatal brain ailments such as mad cow disease and scrapie in animals and, in rare cases, Creutzfeldt-Jacob disease and kuru in humans.

Because similar protein self-replication occurs in neurodegenerative diseases, the findings, published in the Nature, may also help explain the progression of Alzheimer’s, Parkinson’s and Huntington’s diseases.

Prasanna Satpute-Krishnan and Tricia Serio, both in Brown’s Department of Molecular Biology, Cell Biology and Biochemistry, conducted the research using Sup35, a yeast protein similar to the human prion protein PrP.

The researchers tagged a non-prion form of Sup35 with green fluorescent protein in one group of cells and “mated” these cells with another group that contained the prion form.
When the two forms came in contact in the same cell, the green-glowing, healthy protein changed pattern – a visual sign that it converted to the prion form.
These results were confirmed in a series of experiments using different biochemical and genetic techniques.

Because proteins can’t replicate like DNA and RNA, the research helps explain the puzzling process of how prions multiply and spread infection.

Satpute-Krishnan said the speed of protein conversion was surprising. “ The prions were taking all the existing protein and refolding it immediately,” she said.

After the conversion, the yeast cells remained healthy but had new characteristics. This survival supports the theory that prions have endured through evolution because shape-shifting is advantageous, allowing cells to avoid stress by rapidly adjusting to a new environment.

“ Our studies provide some insight into how the appearance of a misfolded protein – a rare event – can lead to devastating neurological diseases,” said Serio. “ Just a small amount of prion-state protein can rapidly convert healthy protein into a pathogenic form.”

Source: Brown University, 2005


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